Document Type : Scientific extension


Assistant Professor. Aerospace Research Institute, Ministry of Science, Research and Technology.Tehran.Iran.


Living organisms have evolved in the presence of constant gravity (1g) on Earth and their growth is related to rate of ribosome, protein biosynthesis, and regulation of cell cycle phases. Microgravity does not have the same effects on different phases of cell cycle such that activity of some phases is increased. It changes the number of cell cycles per time. In the cell growth process, rate of ribosome biosynthesis is decreased under simulated microgravity and a large variation is observed in abundance of nucleolar proteins (Nucleolin and Fibrillarin). Under normal conditions, growth and cell division are coupled, and microgravity uncouples these processes. The study of plant cellular response can help improvement of biological knowledge and preparation of life support systems in space.


[1]  Barlow, P.W., “Gravity Perception in Plants: A Multiplicity of Systems Derived by Evolution?ˮ, Plant, Cell and Environment, Vol. 18, No. 9, 1995, pp. 951–962, 1995.
[2]  Matía, I., Gonzalez-Camacho, F., Herranz, R., and Kiss, J.Z., “Plant Cell Proliferation and Growth Are Altered by Microgravity Conditions in Spaceflightˮ, Journal of Plant Physiology, V0l. 167, No. 3, pp. 184–193, 2010.
[3]  Musk, E., “Making Humans a Multi-Planetary Speciesˮ, New Space, Vol. 5, No. 2, pp. 46–61, 2017.
[4]  Finger, B.W., Lantz, G.A., and Theno, T.W., Mars One Habitat ECLSS (ECLSS) Conceptual Design Assessment, Paragon Space Development Corporation, Michigan, 2015.
[5]  Lasseur, Ch., Paille, C., Lamaze, B., Rebeyre, P., Rodriguez, A., Ordonez, L., Marty, F., “Melissa: The European Project of Closed Life Support Systemˮ, Gravitational and Space Biology, Vol. 23, No. 2, pp. 3–12, 2010.
[6]  Herranz, R. and Medina, F.J., “Cell Proliferation and Plant Development under Novel Altered Gravity Environmentsˮ, Plant Biology, Vol. 16, pp. 23–30, 2014.
[7]  Medina, F.J. and Herranz, R., “Microgravity Environment Uncouples Cell Growth and Cellproliferation in Root Meristematic Cells: The Mediator Role of Auxinˮ, Plant Signaling and Behavior, Vol. 5, No. 2, pp. 176–179, 2010.
[8]  Gerttula, S., Zinkgraf, M., and Groover, A., “Transcriptional and Hormonal Regulation of Gravitropism of Woody Stems in Populusˮ, The Plant Cell-American Society of Plant Biologists, Vol. 27, No. 10, pp. 2800–2813, 2015.
[9]  Chebli, Y. and Geitmann, A., “Gravity Research on Plants: Use of Single-Cell Experimental Modelsˮ, Frontiers in Plant Science, Vol. 2, pp. 1–10, 2011.
[10] Hamant, O. and Haswell, E.S., “Life Behind the Wall: Sensing Mechanical Cues in Plantsˮ, BMC Biology, Vol. 15, No. 1, pp. 59, 2017.
[11]   Dewitte, W. and Murray, J.A.H., “The Plant Cell Cycleˮ, Annual Review of Plant Biology, Vol. 54, No. 1, 2003, pp. 235–264.
[12]   Inzé, D. and De Veylder, L., “Cell Cycle Regulation in Plant Developmentˮ, Annual Review of Genetics, Vol. 40, No. 1, pp. 77–105, 2006.
[13]   Scofield, S., Jones, A., and Murray, J.A.H., “The Plant Cell Cycle in Contextˮ, Journal of Experimental Botany, Vol. 65, No. 10, pp. 2557–2562, 2014.
[14]   De Veylder, L., Beeckman, T., and Inzé, D., “The Ins and Outs of the Plant Cell Cycleˮ, Nature Reviews Molecular Cell Biology, Vol. 8, No. 8, pp. 655–665, 2007.
[15]   Menges, M. and Murray, J.A.H., “Synchronization, Transformation, and Cryopreservation of Suspension-Cultured Cellsˮ, Methods in Molecular Biology, Vol. 323, pp. 45–61, 2006.
[16]   Sáez-Vásquez, J. and Medina, F.J., “The Plant Nucleolusˮ, Advances in Botanical Research, Vol. 47, No. 8, pp. 1–46, 2008.
[17] Thiry, M. and Lafontaine, D.L.J., “Birth of a Nucleolus: The Evolution of Nucleolar Compartmentsˮ, Trends in Cell Biology, Vol, 15, No. 4, pp. 194–199, 2005.
[18]   González-Camacho, F. and Medina, F.J., “The Nucleolar Structure and the Activity of Nopa 100, a Nucleolin-Like Protein, During the Cell Cycle in Proliferating Plant Cellsˮ, Histochemistry and Cell Biology, Vol. 125, No. 1–2, pp. 139–153, 2006.
[19]   Shaw, P.J., Nucleolus, Encyclopedia of LifeSciences (ELS), John Wiley & Sons, Chichester, 2005.
[20]   Montacié, Ch., Durut, N., Opsomer, A., Palm, D., Comella, P., Picart, C., Carpentier, M.Ch., Pontvianne, F., Carapito, Ch., Schleiff, E., and Saez–Vasquez, J., “Nucleolar Proteome Analysis and Proteasomal Activity Assays Reveal a Link Between Nucleolus and 26s Proteasome in a. Thalianaˮ, Frontiers in Plant Science, Vol. 8, No. 10, pp. 1–13, 2017.
[21]   Durut, N. and Sáez-vásquez, J., “Nucleolin: Dual Roles in rDNA Chromatin Transcriptionˮ, Gene, Vol. 556, No. 1, pp. 7-12, 2015.
[22]   De Cárcer, G. and Medina, F.J., “Simultaneous Localization of Transcription and Earlyprocessing Markers Allows Dissection of Functional Domains in the Plant Cell Nucleolusˮ, Journal of Structural Biology, Vol. 128, No. 2, pp. 139–151, 1999.
[23]   Petricka, J.J. and Nelson, T. M., “Arabidopsis Nucleolin Affects Plant Development and Patterningˮ, Plant Physiology-American Society Cell Biology, Vol. 144, No. 1, pp. 173–186, 2007.
[24]   Rodriguez-corona, U., Sobol, M., Rodriguez‐Zapata, L.C., and  Hozak, P., “Fibrillarin From Archaea to Humanˮ, Biology of the Cell, Vol. 107, No. 6, pp. 159–174, 2015.
[25] Shav-Tal, Y., Blechman, J., and Zipori, D., “Dynamic Sorting of Nuclear Components into Distinct Nucleolar Caps During Transcriptional Inhibitionˮ, Molecular Biology of the Cell-American Society Cell Biology, Vol. 16, No. 5, pp. 2395– 2413, 2005.
[26]   Kamal, K.Y., Herranz, R., Van Loon, J.J.W.A., Christianen, P.C.M., and Medina, F.J., “Evaluation of Simulated Microgravity Environments Induced by Diamagnetic Levitation of Plant Cell Suspension Culturesˮ, Microgravity Science and Technology, Vol. 28, No. 3, pp. 309–317, 2016.
[27]   Herranz, R., Valbuena, M.A., Youssef, Kh., and Medina, F.J., “Mechanisms of Disruption of Meristematic Competence by Microgravity in Arabidopsis Seedlingsˮ, Plant signaling & behavior, Vol. 9, No. 4, p. 28289, 2014.
[28]   Kamal, K.Y,, Herranz, R., Van Loon, J.J.W.A., and Medina, F.J., “Simulated Microgravity, Mars Gravity, and 2g Hypergravity Affect Cell Cycle Regulation, Ribosome Biogenesis, and Epigenetics in Arabidopsis Cell Cultures”. Scientific Reports, Vol. 8, pp. 1-16, 2018.
[29]   Manzano, A.I., Herranz, R., Manzano, A., van Loon, J.J.W.A., and Medina, F.J., “Early Effects of Altered Gravity Environments on Plant Cell Growth and Cell Proliferation: Characterization of Morphofunctional Nucleolar Types in an Arabidopsis Cell Culture Systemˮ, Frontiers in Astronomy and Space Sciences, Vol. 3, pp. 1–13, 2016.
[30]   Manzano, A.I., Matia, I., Gonzalez- Camacho, F., Carnero- Diaz, E., and van Loon, J.J.W.A., “Germination of Arabidopsis Seed in Space and in Simulatedmicrogravity: Alterations in Root Cell Growth and Proliferationˮ, Microgravity Science and Technology, Vol. 21, No. 4, pp. 293–297, 2009.
[31]  Mouhamad, R.S., Shallal, H.H., and Al-Daoude, A., “Microgravity Effects on the Growth, Cell Cytology Properties and DNA Alterations of Two Iraqi Local Plantsˮ, Rice Research, Vol. 7, No. 2, pp. 293-297, 2019.