نوع مقاله : یادداشت تحقیقاتی

نویسنده

استادیار، پژوهشگاه هوافضا، وزارت علوم، تحقیقات و فناوری، تهران، ایران

چکیده

نیاز به تحقیقات پایة گیاهی تحت شرایط پیچیدة فضا بارها توسط سازمان‌های فضایی دنیا، از جمله ناسا، عنوان شده است. هدف از پژوهش حاضر بررسی اثرات میکروگراویتی القایی روی رویش و رشد لوله گرده لیلیوم می‌باشد. در این تحقیق، میزان زیست‌پذیری دانه‌های گرده توسط روش رنگ‌آمیزی با استوکارمن تعیین شد. دانه‌های گرده لیلیوم در محیط کشت مایع حاوی 10 درصد سوکروز، 6/1 میلی مول H3BO3، 1 میلی‌مول KCl و 1/0 میلی‌مول CaCl2 (pH=5.7) تحت هر دو شرایط کنترل زمینی و کلینواستت رویش پیدا کردند. پس از اینکوباسیون به مدت دو ساعت، گرده‌ها به منظور بررسی درصد جوانه زنی و جهت رشد لوله گرده مورد مطالعه قرار گرفتند. نتایج نشان داد که درصد یا نرخ رویش دانه‌های گرده تحت شرایط میکروگراویتی نسبت به شرایط کنترل زمینی تمایل رو به افزایش را نشان می‌دهد. به هر حال، طول لوله‌های گرده تحت شرایط میکروگراویتی کاهش می‌یابد.

کلیدواژه‌ها

عنوان مقاله [English]

Effects of Simulated Microgravity on Pollen Germination and Growth of Lily

نویسنده [English]

  • Fateme Mousavi

Assistant Professor, Aerospace Research Institute, Ministry of Science, Research and Technology , Tehran, Iran

چکیده [English]

Basic plant research under complex space conditions is required. this is frequently quoted by the world's space agencies, such as NASA. The aim of the present research is to investigate the effects of simulated microgravity on pollen germination and tube growth of Lily. Pollen viability was ascertained by staining with acetocarmine stain. Pollen grains were germinated on the growing media containing 10% sucrose, 1.6 mM H3BO3, 1 mM Kcl, 0.1 mM CaCl2 (pH=5.7), and were incubated both in 1g and on the clinostat. After incubating for 2 h, pollen grains were studied to count for germinated grains and to determine percent germination and pollen tube length. The germination rate of pollen grains showed a tendency to increase under microgravity condition. However, pollen tube length showed a decrease when incubation was performed in simulated microgravity.

کلیدواژه‌ها [English]

  • Pollen
  • Microgravity
  • Clinostat
  • Life Support System
  • Lily
[1]   Volkmann, D. and Baluska, F., "Gravity: One of the Driving Forces for Evolution", Protoplasma, Vol. 229, pp. 143–148, 2006.
[2]   Garshnek, V., "The Lunar Environment as a Fractional-gravity Biological Laboratory", Acta Astronaut., Vol. 33,  pp. 211–215, 1994.
[3]   Clément, G. and Slenzka, K., Fundamentals of Space Biology — Research on Cells, Animals, and Plants in Space, Space Technology Library, 18, Springer-Verlag, New York, 2006.
[4]   Klaus, D.M., "Clinostats and Bioreactors", Gravitational and Space Biology Bulletin, Vol. 14, pp. 55–64, 2001.
[5]   Hajebrahimi, Z., "3-D Clinostat for Microgravity Simulation in Cellular and Molecular Studies", J. Technology in Aerospace EngineeringVol. 1, pp. 27–33, 2017.
[6]   Kuang, A., Musgrave, M.E., Matthews, S.W., Cummins, D.B., and Tucker, S.C., "Pollen and Ovule Development in Arabidopsis Thaliana Under Space Flight Condition". American J. Botany, Vol. 82, pp. 585–595, 1995               .
[7]   Kuang, A., Popova, A.,  Mc Clure, G., and  Musgrave, M.E.,  "Dynamics of Storage Reserve Deposition During BrassicarapaL. Pollen and Seed Development in Microgravity". International J. Plant Sciences, Vol. 166, pp. 85–96, 2005.
[8]   Musgrave, M.E., Kuang, A., and Matthews, S.W., "Plant Reproduction During Space Flight: Importance of the Gaseous Environment", Planta, Vol. 203, pp. 177–184, 1997.
[9]   Popova, A., Musgrave, M., and Kuang, A., "The Development of Embryos in BrassicarapaL. in Microgravity", Cytology and Genetics, Vol. 43, pp. 89–93, 2009.
[10]  Anushri, V., Dhawan, V., and Srivastava, P. S., "A Protocol for in Vitro Mass Propagation of Asiatic Hybrids of Lily Through Liquid Stationary Culture.", In Vitro Cellular & Developmental Biology-Plant, Vol. 36, No. 5, pp. 383, 2000.
[11]  Brickell, C. and Marc Cathey, H., The American Horticultural Society AZ Encyclopedia of Garden Plants. DK Pub., 2004.
[12]  Germana, M., Chianone, B., Melati, M.R., and Firetto, A.,  "Preliminary Results on the Effect of Magnetic Field on another Culture and Pollen Germination of Citrus Clementina Hort. ex Tan". In: ISHS Acta Horticulturae, Vol. 625, pp. 411 – 418.
[13]  Pertl-Obermeyer, H. and Obermeyer, G., "Pollen Cultivation and Preparation for Proteomic Studies", Methods in Molecular Biology, Vol. 1072, pp. 435-49, 2014.
[14]  De Micco, V., Scala, M., and Aronne, G., "Evaluation of the Effect of Clinostat Rotation on Pollen Germination and Tube Development as a Tool for Selection of Plants in Space", Acta Astronautica, Vol. 58, No. 9, pp. 464-470, 2006.
[15]  De Micco, V., Scala, M., and Aronne, G., "Effects of Simulated Micro- gravity on Male Gametophyte of PrunusPyrus, and Brassica Species", Protoplasma, Vol. 228, pp. 121–126, 2006.
[16]  Chebli, Y. and Geitmann, A., "Gravity Research on Plants: Use of Single-cell Experimental Models.", Frontiers in plant science, Vol. 2, pp. 56, 2011.
[17]  Pertl-Obermeyer, H. and Obermeyer, G., "Pollen Cultivation and Preparation for Proteomic Studies", Methods in molecular biology, Vol. 1072, pp. 435-49, 2014.
[18]  Musgrave, M. E., Kuang, A., Allen, J., Blasiak, J., and van Loon, J.J.W.A., "Brassica rapa L. Seed Development in Hyper Gravity.", Seed Science Research, Vol. 19, No. 2, pp. 63-72, 2009.
[19]  Musgrave, M., Kuang, A., Allen, J., and van Loon, J.J.W.A., "Hyper Gravity Prevents Seed Production in Arabidopsis by Disrupting Pollen Tube Growth.", Planta, Vol. 230, No. 5, pp. 863-870, 2009.
[20]  Lisboa, Y.S., Scherer, G.E.F., and Quader, H., "Endocytosis in Tobacco Pollen Tubes: Visualization and Measurement of Plasma Membrane Retrieval During Different Gravity Conditions Indicates Gravity-Dependence of Endocytosis.", Life in Space for Life on Earth, Vol. 501, pp. 397-398, 2002.